Coding

Part:BBa_K2144011:Design

Designed by: Oskar hman   Group: iGEM16_Stockholm   (2016-10-13)


Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To insert the Linker-tag sequence overhang PCR was performed


Source

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References